Chloroplast Ubiquitin E3 Ligase SP1: Does It Really Function in Peroxisomes?1[CC-BY]

Dear Editor, Pan et al. (2016) presented evidence that the plant ubiquitin E3 ligase SP1 directs proteasome-mediated regulation of the peroxisomal protein import machinery. This was surprising, as SP1 was originally identified as a chloroplast outer envelope-localized regulator of the chloroplast protein import machinery (Ling et al., 2012; Ling and Jarvis, 2015), and there was nothing in the original datasets to suggest functions elsewhere in the cell. It was also difficult to understand how a key regulator of chloroplast protein import, which mediates nuanced and selective effects during development and under stress (Ling et al., 2012; Ling and Jarvis, 2015), could additionally operate in a second organelle with a very different protein import system (Léon et al., 2006). We sought to address these issues by conducting additional experimental analyses. The veracity of the model proposed by Pan et al. (2016) depended heavily on qualitative immunoblot data showing opposing effects on the abundance of peroxisomal protein import apparatus components (PEX13 and PEX14) in sp1 mutant and SP1 overexpressor plants. This was because much of the other data presented in support of the model were either physiological measurements of a general nature that cannot be conclusively linked to peroxisomes (i.e. hypocotyl and root growth, which are both known to be influenced by chloroplast protein import; Huang et al., 2011) or analyses of fusion proteins with large tags transiently overexpressed in a heterologous system for which physiological relevance cannot be assured (Dixit et al., 2006; Tanz et al., 2013). Owing to the critical importance of the PEX protein abundance data, we sought to reproduce them in quantitative fashion using plants equivalent to those analyzed previously (Pan et al., 2016). However, in repeated experiments, we observed no significant changes in the levels of either PEX protein in response to SP1 manipulation, relative to thewild type (Fig. 1, A and B). In contrast, the levels of chloroplast TOC proteins, which are well-established targets of SP1, were significantly changed in both genotypes (Fig. 1, A and B), in line with previous observations (Ling et al., 2012; Ling and Jarvis, 2015); this clearly demonstrated the sensitivity of our assay conditions. Next, we reassessed the subcellular localization of SP1. We began by analyzing an SP1-YFP fusion by confocalmicroscopy in stably transformedArabidopsis plants and in leaves transiently transformed by agrobacterial infiltration. However, YFP signals were not detectable in these experiments (Fig. 1, C and D; data not shown), so we concluded that SP1(-YFP) protein abundance is suppressed by stringent (auto)regulation, as is typical for ubiquitin E3 ligases. Our inability to detect YFP signals akin to those described by Pan et al. (2016) suggested that the cells in the previous study may have overaccumulated SP1-YFP to unusually high levels for some reason. To aid detection of SP1-YFP, we next used transient protoplast transfection, which achieves overexpression by greatly increasing gene copy-number per cell. Over three independent experiments, we analyzed 75 cells displaying clear SP1-YFP fluorescence. Of these, the majority displayed fluorescence that was obviously restricted to the chloroplast envelope (Fig. 1E, top row). Nonetheless, approximately 8% of cells (six in total) did show a degree of extrachloroplastic fluorescence, some of which appeared to be associated with the peroxisomes (Fig. 1E, middle row). It is well documented that the overaccumulation of a protein can force itsmislocalization (Dixit et al., 2006; Tanz et al., 2013).Moreover, cells possess quality control systems to eliminate mistargeted proteins, suggesting that minor mislocalization may not be an uncommon event even under native conditions (Chen et al., 2014). Thus, while the possibility that more extensive SP1-peroxisome association occurs in other cell types could not be excluded, we suspected that the rare peroxisomal signals detected inmesophyll protoplasts were the result of mislocalization caused by overexpression. To assess whether the tendency of SP1-YFP to occasionally associate with peroxisomes in transfected protoplasts is a unique feature of SP1, or a more general phenomenon linked to overexpression, we similarly analyzed protoplasts expressing a YFP-Toc33 fusion. Over more than 2 decades of research, Toc33 has been clearly established as a core component of the chloroplast protein import machinery with no suggested functions elsewhere in the cell (Paila et al., 2015). Most YFP-Toc33-expressing cells showed only chloroplast envelope fluorescence, but, as with SP1-YFP, a small proportion of cells (approximately 13% of those analyzed) unexpectedly displayed peroxisomal signals (Fig. 1E, bottom row). Thus, we concluded that the microscopy data linking SP1 to peroxisomes (Pan et al., 2016) are of questionable physiological relevance. Pan et al. (2016) also used subfractionation to support their contention that SP1 is in peroxisomes, but these data too are equivocal owing to their reliance on an overexpressed, tagged protein for detection, and the fact the protocol used is described by its authors as being unable to yield pure peroxisome fractions devoid of chloroplast contamination (Reumann and Singhal, 2014; Reumann and Lisik, 2017). 1 This work was supported by the Biotechnology and Biological Sciences Research Council (grant no. BB/N006372/1). 2 These authors contributed equally to the article. 3 Address correspondence to [email protected]. Article free via Creative Commons CC-BY 4.0 license. www.plantphysiol.org/cgi/doi/10.1104/pp.17.00948